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Phis2

Webb酵母单杂. 使用pHIS2载体,将启动子序列构建在HIS3报告基因前面的多克隆位点上,对应的转录因子构建在AD载体上即可。. 使用Y187菌株,它相对于AH109菌株在基因组上少 … Webb1 jan. 2016 · This paper reports on research carried out in the field of Human-Computer Interaction (HCI) on the interaction between a human and a mobile device, in…

ATDOF5.8 protein is the upstream regulator of - ScienceDirect

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酵母杂交实验常见问题——单杂知识及自激活检测篇

Webb12 mars 2024 · The pHIS2-MdANSPro and the pHIS2-MdUFGTPro vectors were respectively transformed into Y187 yeast strains that were cultivated in -T-H selective media containing different concentrations of 3-AT. Reduced yeast growth was observed with increasing 3-AT concentration, and yeast could not grow on -T-H selective media … Webbphis-docs-community Overview. This repository contains OpenSILEX PHIS users documentation. Background. PHIS (Phenotyping Hybrid Information System) is … WebbY187-pHis2系统酵母单杂原理:当猎物蛋白(Prey)结合到诱饵序列(Bait DNA)上,GAL4 AD就会激活下游报告基因HIS3的表达,能够在含有3-AT的培养基上生长,从而确定DNA和蛋白有相互。. 本方案中检测诱饵酵母菌株的3-AT最佳使用浓度和互作验证都采用了稀释点板的方法 ... photo text art

pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

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Phis2

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Phis2

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http://www.honorgene.com/product_list/carrier_library/123112.html Webb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls.

Webb1 apr. 2024 · To investigate the binding of TaMYB44-D to starch biosynthesis-related genes, the promoter sequences of 12 selected genes were cloned in front of the reporter gene HIS3 to construct the pHIS2.1-Pro plasmids. The plasmids pHIS2.1-Pro and pGADT7-Sec2-TaMYB44-D were co-transfected into the Y187 yeast strain to perform the Y1H … WebbStack Exchange network consists of 181 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, …

WebbY187-pHis2YeastOne-HybridMediaKit(酵母单杂培养基套装YM1010),含有用于Y187-pHis2系统 酵母单杂交文库筛选的全套培养基,性价比更高。培养基以非常方便使用的 … Webb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors were transformed into the yeast strain Y187 for subsequent growing on − Trp/−His (−T/−H) medium with different concentrations of 3-AT.

WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …

WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … photo text convert to wordWebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … photo text appWebb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and … photo text changerWebbpHis1, pHis2, pHis3 pGST1, pGST2, pGST3 pMBP1, pMBP2, pMBP3: pET44a-c: Novagen: T7 promoter, N-term his tag followed by NusA fusion protein, another his-tag, S-tag, … how does synchronous rectification workWebb18 okt. 2024 · 载体描述:. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a … photo text app downloadWebb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors … how does synchrony financing workWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … photo text editing apps