Glutamax thermo fisher
WebOpti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO 2 ... WebRelated Products: 10566 - DMEM, high glucose, GlutaMAX(TM) Cat No: 10566016 , 10566024 , 10566032
Glutamax thermo fisher
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WebGibco™ L-glutamine is a ready-to-use 200 mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the … WebFor overexpression studies, HEK293T cells were maintained in high-glucose (4.5 g/L) DMEM GlutaMax (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA) and transfected (at ~80–90% confluency) with pri-miRNA expression vectors by using polyethyleneimine …
WebGibco™ GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. WebViability was assessed at day 3 of culture for scaffolds seeded with U87 MG cells and day 7 for astrocytes and cocultured cells. Cells were incubated at 21 °C for 30 min with Calcein-AM (1:500, green/living cells; Thermo Fisher Scientific, Waltham, MA) and Ethidium Homodimer (1:1000, red/dead cells; Sigma-Aldrich, St. Louis, MO) diluted in PBS.
WebApr 8, 2024 · Forward programming was initiated 24 h after the split. The induction was performed in DMEM/F-12 supplemented with Glutamax (100×), Non-Essential Amino Acids (100×), 50 μM 2-Mercaptoethanol (Thermo Fisher Scientific), 1% Penicillin/Streptomycin, 1 … WebGLUTAMAX I eliminates problems associated with the spontaneous breakdown of Lglutamine during incubation. It is highly soluble in aqueous solution and is heat stable. It is supplied as a 200mM (100X), liquid stock …
WebThe medium was prepared with 45% Dulbecco’s modified eagle medium: nutrient mixture F-12 (DMEM/F12; Sigma Aldrich, St Louis, MO), 45% Neurobasal (Thermo Fisher Scientific), 0.5% N2 supplement (Thermo Fisher Scientific), 1% B27 supplement (Thermo Fisher Scientific), 1% GlutaMAX, 1% nonessential amino acids (Sigma Aldrich), 0.1 mM β ...
Web(+) Glutamax (TM) A41240 2024-02 2 to 8C Storage Instructions: Protect from light Storage Temp: Expiration Date: Item Number: Lot Number: 2482248 TEST TEST ID SPECIFICATION RESULT UNITS Endotoxin Testing ENDO0007 <=1.00 <0.10 EU/mL LV-MAX Performance Assay LVMAX0001 Acceptable Acceptable Mycoplasma qPCR … med rhonchi meaningWebGlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX™ Supplement: • Minimizes toxic ammonia build-up. • Improves cell viability and growth. • Remains stable across a wide range of temperatures. med rhein testsWebDescription Suitable for both adherent and suspension culture of mammalian cells, with no adaptation required Offered as a 200mM L … naked petite heat eyeshadow palette