Cytiva glutathione sepharose 4b说明书
Web丁香通为您提供GLUTATHIONES商品详情介绍:价格:¥3046,货号:17075601,品牌:Cytiva,产地:美国,供应商:Cytiva,详见丁香通GLUTATHIONES商品详情页; ... GLUTATHIONE SEPHAROSE 4B 10 ML ... 谷胱甘肽Sepharose 4B是一种亲和色谱树脂,用于批量纯化GST标记的蛋白质, 并且此时需要 ... WebGST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
Cytiva glutathione sepharose 4b说明书
Did you know?
WebFor ordering information, visit cytiva.com istidine-tag GST-tag Strep-tag II M-tag AC Recombinant tagged proteins igh binding capacity is your priority You need scalability igh resolution ... Glutathione Sepharose 4 Fast Flo repacked irap column rap . 4 Affinity chromatography—antibodies and antibody fragments WebOVERVIEW. Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required. High capacity, single-step glutathione S-transferase (GST) tagged protein purification. Supplied as pre-swollen bulk (loose) resin. Chemically stable with all commonly used aqueous …
Web6 Incubate the cleared supernatant with 2 ml of Glutathione Sepharose 4B beads slurry (Cytiva) ... Cytiva) pre-equillibrated with a SEC buffer containing 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, and 1 ... WebGlutathione Sepharose 4B (Cytiva) Superdex 200 increase 10/300 column (Cytiva) ... Cytiva) pre-equillibrated with a SEC buffer containing 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 1 mM DTT. ...
WebGST tag cleavage was performed by incubation with PreScission™ Protease (Cytiva) in 50 mM Tris, 100 mM NaCl, 1 mM EDTA, 5 mM DTT pH 7.4 O/N at 4 °C. The solution was loaded again on the glutathione Sepharose ® 4B column to separate the PDZ3 domain from cleaved GST. Buffers used for the final equilibration of PSD-95 PDZ3 are the same ... WebPress Release. AcuraBio、cGMPプラスミドDNA CDMOサービスを拡大、Cytivaの最新シングルユース精製技術でmRNAおよび細胞・遺伝子治療薬の供給制約を軽減(23.02.10). お知らせ. 先着2,000名様!. 毎年人気の卓上カレンダーお申込み受付中(23.1.19). Press Release. Cytiva、CEVEC ...
http://img1.bioon.com/reagent/Show_product.asp?id=62744
WebGlutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins. Proteins are eluted under … ctr boise idahoWebGlutathione Sepharose ® 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins. GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose ® 4B. Proteins are eluted under mild, … earth syscom pvt ltdWebGlutathione Sepharose ® 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion … ctr boonie hatWebJan 28, 2024 · [email protected]. Call/Text: (571) 461-1700 . PureDropIV HQ. 6369 Rolling Mill Place. Suite 101. Springfield, VA 22152 earth system dynamics 影响因子WebGlutathione Sepharose 4B,100 mL Glutathione Sepharose 4B 是一种亲和层析填料,用于批量纯化带 GST 标签的蛋白质,且结合载量较高。 更改产品 ctr boosterWeb1. Determine the medium volume of Glutathione Sepharose 4B required for your purification. Note: Glutathione Sepharose 4B is delivered in 20% ethanol. Prepare a 50% slurry for your purification. 2. Gently shake the bottle of Glutathione Sepharose 4B to resuspend the slurry. 3. Use a pipette or measuring cylinder to remove sufficient slurry … ctr bota foraWeb1.2 Gently shake the bottle of Glutathione Sepharose 4B to resus-pend the gel. 1.3 Use a pipet to remove sufficient slurry for use and transfer to an appropriate container/tube. Dispense 1.33 ml of the original Glutathione Sepharose 4B slurry per ml of bed volume required. One ml of drained gel is capable of binding at least 8 mg of recombinant ... earth system dynamics期刊缩写