Cisplatin cck8

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August undefined, 2024

WebAug 8, 2024 · a Before and after treatment with the supernatants of Tregs, cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM) was analyzed by the CCK8 assay. b With different concentrations of Treg supernatants (supernatant frequency of Treg/tumor cell = 1:1, 1:2 and 1:3), cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM ... WebJan 12, 2024 · Parental and cisplatin-resistant HeLa cells were cultured in the presence of 1.0 μg/mL cisplatin in 96-well plates and stained with cell counting kit-8 (CCK8) at indicated time. Values are presented relative to cell viability on day 0. * p < 0.05, ** p < 0.01 compared with the parental cells; ( F ) Western blotting analysis of NHERF1 ...

Cisplatin (Cisplatin Injection): Uses, Dosage, Side Effects

WebObjective: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin. Methods: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to … diamond and topaz bracelet

HMG20A was identified as a key enhancer driver …

Web目的探讨姜黄挥发油(TVO)联合顺铂对人皮肤鳞状细胞癌(鳞癌)A431细胞增殖、凋亡的影响及机制.方法取对数生长期A431细胞,用5、10、20、40、80 mg/L TVO处理,对照组用含有1％二甲基亚砜(DMSO)的DMEM高糖培养基处理,24 h后,CCK8法检测细胞增殖情况.另取部分A431细胞,分为4组,分别用含1％ DMSO的DMEM高糖培养基(对照 ... http://www.rx-8.com/chanpinzhongxin/203370.html WebFeb 28, 2024 · CCK8 was used in ( a) A549 cells and ( b) H460 cells with different concentrations of cisplatin. ( c) CCK8 was used in A549 and H460 cells transfected with miR-494 or controlled with... diamond and tio2 composites

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WebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the … WebMar 15, 2024 · In vivo experiment showed that pretreatment with anemoside B4 effectively adjusted body weight and kidney index, and reduced cisplatin-elevated blood urea nitrogen (BUN) and creatinine (CREA) levels, as well as ameliorated the histopathological damage. diamond and turquoise bandWebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, ... Cell counting kit-8 assay. Cell viability was measured using the CCK-8 (SAB Biotech., College Park, MD, USA) assay. Cells were cultured in 96-well plates at 3 × 10 3 cells/well for 12 h. Following treatment for 48 h, the CCK-8 reagent was ... circle k in brunswick

"WebWe established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827). The EGFR-related signals were examined by Western blotting. MTT assay and the trypan blue exclusion method were used for the in vitro study, while tumor size and the SUV of the 18FDG-PET scans were … " - Cisplatin cck8

Cisplatin cck8

Exosome-transmitted circVMP1 facilitates the progression and cisplatin …

WebMay 27, 2024 · Uncovering the role of p62 in the development of cisplatin resistance in ovarian cancer. ... (IHC) and its proliferation and invasion effects via CCK8 and transwell assays. Additionally, expression of cyclin D1, MMP2, p‐ERK, and EMT‐related proteins was measured by western blotting. We found that SIX1 had significantly higher expression in ... WebMar 27, 2024 · Cisplatin-induced cell killing was analyzed by CCK8 assay. Intracellular ROS levels were detected by fluorescence-based flow cytometry analysis. In vitro overexpression and knockdown of GPX1 expression were performed using GPX1 expression vector and siRNA approaches.

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WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … WebCCK8 assays were also utilized to detect IC50 values for cisplatin and 5-Fu. Results: The expression of circNRIP1 was significantly increased in the serum of chemoresistant NPC patients. At a functional level, we determined that circNRIP1 is able to sequester miR-515-5p, thereby inhibiting its ability to post-transcriptionally suppress IL-25 ...

WebCisplatin-induced acute kidney injury in mice was established by intraperitoneal injection of cisplatin. The levels of urea nitrogen and creatinine in serum were measured， renal pathology change was assayed by HE staining， qRT-PCR and Western blot were used to detect the expression levels of PIM1 mRNA and PIM1 protein. WebApr 9, 2024 · Then cells were treated with cisplatin as indicated concentrations. 24 or 48 h later, 10 μL CCK8 reagent (HY-K0301, MedChemExpress, USA) was added into each well for another 2 h. Then the absorbance was measured at 490 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader (Bio Tek).

WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ... Web瑞禧生物提供科研级cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购cisplatin-PEG-NPC顺铂-聚乙二醇-硝基 ...

WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 …

WebSep 9, 2016 · The CCK8 assays revealed that, compared to scramble vector, PD-L1 depletion (Fig 2C, left panel) induced more cell growth arrest in the presence of cisplatin (Fig 2C, right panel), suggesting that PD-L1 abrogation restored the sensitivity of H69R and H82R to cisplatin treatment. Thus, PD-L1 upregulation might be required for H69R and … diamond and tionda bradley foundWebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often … circle k in bucyrus ohioWebJul 4, 2024 · To investigate whether miR‐320a regulates laryngeal carcinoma cell cisplatin resistance, miR‐320a overexpression HEp‐2 cells were treated with various concentration of cisplatin. CCK8 assays demonstrated that miR‐320a overexpression significantly inhibited HEp‐2 cell viability (Figure 4 E). diamond and time aucklandWebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of... diamond and triangleWebCisplatin is a chemotherapy drug that contains the metal platinum. It stops or slows growth of cancer cells and other cells that grow rapidly by damaging their DNA. FDA Approved … diamond and turquoise jewelryWebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was … circle k in clinton iowaWebJan 1, 2024 · New therapy targets are urgently needed to reverse DDP resistance. Methods: The protein levels of these molecules were analyzed by Western blotting and immunofluorescent staining. Cell proliferation was detected by CCK8 and MTT assays. Cell migration and invasion were analyzed by wound healing assay and Transwell assays. diamond and tungsten carbide